Epigenetic markers of tooth eruption – DNA methylation and histone acetylation-Reference-Cited by-同舟云学术

Epigenetic markers of tooth eruption – DNA methylation and histone acetylation

Published:2024-07-16 Issue:4 Volume:132 Page:
ISSN:0909-8836
Container-title:European Journal of Oral Sciences
language:en
Short-container-title:European J Oral Sciences

Author:

Westerlund Anna¹^ORCID,Shikhan Asal²^ORCID,Sabel Nina³^ORCID,Asa'ad Farah⁴^ORCID,Larsson Lena⁴^ORCID

Affiliation:

1. Department of Orthodontics, Institute of Odontology, The Sahlgrenska Academy University of Gothenburg Gothenburg Sweden

2. Department of Periodontology, Institute of Odontology, The Sahlgrenska Academy University of Gothenburg Gothenburg Sweden

3. Department of Pediatric Dentistry, Institute of Odontology, The Sahlgrenska Academy University of Gothenburg Gothenburg Sweden

4. Department of Oral Biochemistry, Institute of Odontology, The Sahlgrenska Academy University of Gothenburg Gothenburg Sweden

Abstract

AbstractThe present study aimed to evaluate whether epigenetic markers are expressed in the dental follicles surrounding ectopically erupting teeth. Twenty‐one dental follicles were collected in 20 adolescent children through surgical exposure of ectopic teeth. The epigenetic modifications of DNA methylation and histone acetylation were evaluated by immunohistochemistry. The results showed cells positive for DNA‐methyltransferase 1 (DNMT1), DNA methyltransferase 3 beta (DNMT3B), ten‐eleven translocation‐2 (TET2), acetyl‐histone H3 (AcH3), acetyl‐histone H4 (AcH4), 5‐methylcytosine (5mC), and 5‐hydroxymethylcytosine (5hmC) were present in all the samples. The levels of epigenetic markers representing active chromatin (5hmC, AcH3, AcH4, and TET2) were statistically significantly higher than those of markers representing inactive chromatin (5mC, DNMT3B, DNMT1). In conclusion, follicles in ectopic teeth display major epigenetic modifications. In the follicles, epigenetic markers associated with the activation of bone‐related genes are more abundant than markers associated with the inactivation of bone‐related genes.

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/eos.13005

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