The impact of active cytomegalovirus infection on donor‐derived cell‐free DNA testing in heart transplant recipients

Author:

Alam Amit H.1,Van Zyl Johanna23ORCID,Shakoor Hira I.3,Farsakh Dana3,Abdelrehim Ahmad B.3,Maliakkal Neville3,Jamil Aayla K.23ORCID,Patel Raksha4,Felius Joost23ORCID,McKean Staci4,Hall Shelley A.24ORCID

Affiliation:

1. Division of Cardiology New York University Langone Health New York New York USA

2. Texas A&M University Health Science Center College of Medicine Dallas Texas USA

3. Baylor Scott & White Research Institute Baylor Scott & White Health Dallas Texas USA

4. Center for Advanced Heart and Lung Disease Baylor University Medical Center Baylor Scott & White Health Dallas Texas USA

Abstract

AbstractBackgroundLittle is known about the relationship between cytomegalovirus (CMV) infections and donor‐derived cell‐free DNA (dd‐cfDNA) in heart transplant recipients.MethodsIn our study, CMV and dd‐cfDNA results were prospectively collected on single‐organ heart transplant recipients. If the CMV study was positive, a CMV study with dd‐cfDNA was repeated 1‐3 months later. The primary aim was to compare dd‐cfDNA between patients with positive and negative CMV results.ResultsOf 44 patients enrolled between August 2022 and April 2023, 12 tested positive for CMV infections, 25 were included as controls, and seven patients with a viral infection without CMV were excluded. Baseline characteristics did not differ significantly between CMV‐positive and CMV‐negative patients with the exception of a later median time post‐transplant in the CMV‐positive group (253 days vs. 120 days, p = .03). Dd‐cfDNA levels were significantly higher in patients with CMV infections compared to those without (p < .001) with more patients in the CMV positive group showing dd‐cfDNA results ≥.12% (75% vs. 8%, p < .001) and ≥.20% (58% vs. 8%, p = .002). Each 1 log10 copy/ml reduction in CMV viral load from visit 1 to visit 2 was associated with a.23% reduction in log10 dd‐cfDNA (p = .002).ConclusionOur findings suggest that active CMV infections may raise dd‐cfDNA levels in patients following heart transplantation. Larger studies are needed to validate these preliminary findings.

Publisher

Wiley

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