Affiliation:
1. Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Ghent Belgium
2. VIB Center for Plant Systems Biology 9052 Ghent Belgium
3. State Key Laboratory of Crop Genetics and Germplasm Enhancement and MOA Key Laboratory of Plant Nutrition and Fertilization in Lower‐Middle Reaches of the Yangtze River Nanjing Agricultural University Nanjing 210095 China
4. INRAE, CNRS LIPME, Université de Toulouse F‐31326 Castanet‐Tolosan France
Abstract
SUMMARYLateral roots are crucial for plant growth and development, making them an important target for research aiming to improve crop yields and food security. However, their endogenous ontogeny and, as it were, stochastic appearance challenge their study. Lateral Root Inducible Systems (LRIS) can be used to overcome these challenges by inducing lateral roots massively and synchronously. The combination of LRISs with transcriptomic approaches significantly advanced our insights in the molecular control of lateral root formation, in particular for Arabidopsis. Despite this success, LRISs have been underutilized for other plant species or for lateral root developmental stages later than the initiation. In this study, we developed and/or adapted LRISs in rice, Medicago, and Arabidopsis to perform RNA‐sequencing during time courses that cover different developmental stages of lateral root formation and primordium development. As such, our study provides three extensive datasets of gene expression profiles during lateral root development in three different plant species. The three LRISs are highly effective but timing and spatial distribution of lateral root induction vary among the species. Detailed characterization of the stages in time and space in the respective species enabled an interspecies co‐expression analysis to identify conserved players involved in lateral root development, as illustrated for the AUX/IAA and LBD gene families. Overall, our results provide a valuable resource to identify potentially conserved regulatory mechanisms in lateral root development, and as such will contribute to a better understanding of the complex regulatory network underlying lateral root development.
Funder
European Molecular Biology Organization
Fonds Wetenschappelijk Onderzoek
Ministry of Science and Technology of the People's Republic of China
Subject
Cell Biology,Plant Science,Genetics
Cited by
1 articles.
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