Improved multiplex TaqMan qPCR assay with universal internal control offers reliable and accurate detection of Clavibacter michiganensis-Reference-Cited by-同舟云学术

Improved multiplex TaqMan qPCR assay with universal internal control offers reliable and accurate detection of Clavibacter michiganensis

Published:2021-02-23 Issue:3 Volume:131 Page:1405-1416
ISSN:1364-5072
Container-title:Journal of Applied Microbiology
language:en
Short-container-title:J Appl Microbiol

Author:

Ramachandran S.¹²,Dobhal S.³,Alvarez A.M.³,Arif M.³^ORCID

Affiliation:

1. Foreign Disease and Weed Science Research Unit USDA‐ARS Fort Detrick MD USA

2. ARS Research Participation Program Oak Ridge Institute for Science and Education Oak Ridge TN USA

3. Department of Plant and Environmental Protection Sciences University of Hawaii at Manoa Honolulu HI USA

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/jam.15017

Reference55 articles.

1. Real-Time PCR for Diagnosing and Quantifying Co-infection by Two Globally Distributed Fungal Pathogens of Wheat

2. Primers with 5′ flaps improve real-time PCR

3. One lab‐one protocol: synergetic effect of 5’AT‐rich flap to harmonize qPCR protocols for easy, sensitive and cost‐effective diagnostics. (Abstr.);Arif M.;Phytopathology,2019

4. Primer Modification Improves Rapid and Sensitive In Vitro and Field-Deployable Assays for Detection of High Plains Virus Variants

5. Comparative Assessment of 5′ A/T-Rich Overhang Sequences with Optimal and Sub-optimal Primers to Increase PCR Yields and Sensitivity

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