Affiliation:
1. Department of Neurobiology, Institute for Biological Research “Sinisa Stankovic” ‐ National Institute of Republic of Serbia University of Belgrade Belgrade Serbia
2. NanoLab, Biomedical Engineering, Faculty of Mechanical Engineering University of Belgrade Belgrade Serbia
3. TFT Nano Center Belgrade Serbia
4. Department of Molecular Biology and Endocrinology, “VINČA” Institute of Nuclear Sciences ‐ National Institute of Republic of Serbia University of Belgrade Belgrade Serbia
Abstract
AbstractIntroductionIn the present study, we assessed the effects of the hyper‐harmonized‐hydroxylated fullerene–water complex (3HFWC) on Alzheimer's disease (AD) neuropathological hallmarks in 5XFAD mice, an AD animal model.MethodsThe 3‐week‐old 5XFAD mice were exposed to 3HFWC water solution ad libitum for 3 months in the presymptomatic phase of pathology. The functional effects of the treatment were confirmed through near‐infrared spectroscopy (NIRS) analysis through machine learning (ML) using artificial neural networks (ANNs) to classify the control and 3HFWC‐treated brain tissue samples. The effects of 3HFWC treatment on amyloid‐β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in cortical and hippocampal tissue were assessed.ResultsThe 3HFWC treatment significantly decreased the amyloid‐β plaque load in specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not induce the activation of glia (astrocytes and microglia) nor did it negatively affect synaptic protein markers (GAP‐43, synaptophysin, and PSD‐95).ConclusionThe obtained results point to the potential of 3HFWC, when applied in the presymptomatic phase of AD, to interfere with amyloid plaque formation without inducing AD‐related pathological processes such as neuroinflammation, gliosis, and synaptic vulnerability.
Funder
Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
Subject
Pharmacology (medical),Physiology (medical),Psychiatry and Mental health,Pharmacology
Cited by
1 articles.
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