Affiliation:
1. Department of Applied Plant Science, College of Agriculture and Life Sciences Chonnam National University Gwangju 61186 Korea
2. Interdisciplinary Program in IT‐Bio Convergence System Chonnam National University Gwangju 61186 Korea
3. Laboratoire Reproduction et Développement des Plantes Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE Lyon F‐69342 France
Abstract
Summary
Production of in planta haploid embryos that inherit chromosomes from only one parent can greatly increase breeding efficiency via quickly generating homozygous plants, called doubled haploid. One of the main players of in planta haploid induction is a pollen‐specific phospholipase A, which is able, when mutated, to induce in vivo haploid induction in numerous monocots. However, no functional orthologous gene has been identified in dicots plants.
Here, we show that loss‐of‐function of gynoecium‐expressed phospholipase AII (pPLAIIγ) triggers maternal haploid plants in Arabidopsis, at an average rate of 1.07%. Reciprocal crosses demonstrate that haploid plants are triggered from the female side and not from the pollen, and the haploid plants carry the maternal genome.
Promoter activity of pPLAIIγ shows enriched expression in the funiculus of flower development stages 13 and 18, and pPLAIIγ fused to yellow fluorescent protein reveals a plasma‐membrane localization
Interestingly, the polar localized PIN1 at the basal plasma membrane of the funiculus was all internalized in pplaIIγ mutants, suggesting that altered PIN1 localization in female organ could play a role in maternal haploid induction.
Funder
National Research Foundation of Korea
Rural Development Administration
Cited by
5 articles.
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