ArabidopsisSRPKII family proteins regulate flowering via phosphorylation of SR proteins and effects on gene expression and alternative splicing

Author:

Wang Tongtong1,Wang Xiaofeng1,Wang Haiyan1,Yu Chao1,Xiao Chengyun1,Zhao Yiwu1,Han Huanan1,Zhao Shuangshuang1,Shao Qun1,Zhu Jianhua2,Zhao Yanxiu1,Wang Pingping1,Ma Changle1ORCID

Affiliation:

1. Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences Shandong Normal University Wenhua East Road 88 Jinan Shandong 250014 China

2. Department of Plant Science and Landscape Architecture University of Maryland College Park MD 20742 USA

Abstract

Summary Alternative splicing of pre‐mRNAs is crucial for plant growth and development. Serine/arginine‐rich (SR) proteins are a conserved family of RNA‐binding proteins that are critical for both constitutive and alternative splicing. However, how phosphorylation of SR proteins regulates gene transcription and alternative splicing during plant development is poorly understood. We found that the Arabidopsis thaliana L. SR protein‐specific kinase II family proteins (SRPKIIs) play an important role in plant development, including flowering. SRPKIIs regulate the phosphorylation status of a subset of specific SR proteins, including SR45 and SC35, which subsequently mediates their subcellular localization. A phospho‐dead SR45 mutant inhibits the assembly of the apoptosis‐and splicing‐associated protein complex and thereby upregulates the expression of FLOWERING LOCUS C (FLC) via epigenetic modification. The splicing efficiency of FLC introns was significantly increased in the shoot apex of the srpkii mutant. Transcriptomic analysis revealed that SRPKIIs regulate the alternative splicing of c. 400 genes, which largely overlap with those regulated by SR45 and SC35‐SCL family proteins. In summary, we found that Arabidopsis SRPKIIs specifically affect the phosphorylation status of a subset SR proteins and regulate the expression and alternative splicing of FLC to control flowering time.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

Plant Science,Physiology

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