Metabolic effects of radiation on red blood cells from cold stored whole blood

Author:

Becerra Sandra C.1,Christy Barbara A.23ORCID,Herzig Maryanne C.2,Bynum James A.24,Darlington Daniel N.24

Affiliation:

1. Pathology, Epidemiology and Biostatistics United States Army Institute of Surgical Research Fort Sam Houston Texas 78234 USA

2. Blood and Shock Research United States Army Institute of Surgical Research Fort Sam Houston Texas 78234 USA

3. Department of Molecular Medicine UT Health San Antonio San Antonio Texas 78229 USA

4. Department of Surgery UT Health San Antonio San Antonio Texas 78229 USA

Abstract

AbstractBackgroundThe risk of military and civilian radiation exposure is increasing, and determining the effects of exposure is a high priority. Irradiation of the nearby blood supply after a nuclear event may impede mobilization of blood products for resuscitation at a time of great need. RBCs are administered to patients with trauma and hemorrhage to transport and deliver oxygen and avoid tissue hypoxia. Here we determine the effects of ionizing radiation on the energy metabolome of RBCs isolated from cold stored whole blood to determine if their stability is compromised by radiation exposure.Study Design and MethodsWhole blood from healthy volunteers was subjected to 0, 25, or 75 Gy of X‐irradiation, and stored at 4°C. RBCs were isolated from stored WB at 0, 1, 7, 14, and 21 days of storage. The levels of extracted Krebs cycle intermediates, nicotinamide adenine dinucleotides, and phosphorylated derivatives of adenosine and guanosine were determined by tandem mass spectroscopy.ResultsIrradiation at either 25Gy or 75Gy had no significant effect on any parameter measured compared to control (0Gy). However, there was a significant change over time in storage for ATP, GDP, and guanosine.DiscussionIrradiation at doses up to 75Gy had no effect on the energy metabolome of RBCs prepared from blood stored at 4°C for up to 21 days, suggesting that the RBC energy metabolome is not affected by radiation exposure and the blood can still be used for resuscitation in trauma patients.

Funder

Congressionally Directed Medical Research Programs

Publisher

Wiley

Subject

Hematology,Immunology,Immunology and Allergy

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