Affiliation:
1. Department of Urology the First Affiliated Hospital of Anhui Medical University Anhui Medical University Hefei Anhui China
2. Institute of Urology Anhui Medical University Hefei Anhui China
3. Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University Hefei Anhui China
4. Department of Urology Shanghai East Hospital, School of Medicine Tongji University Shanghai China
5. Department of Urology Southern University of Science and Technology Hospital Shenzhen China
Abstract
AbstractObjectiveTo investigate the mechanism of the CXCL10/CXCR3 axis regulating Th1 cell differentiation and migration through the PI3K/AKT pathway in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).MethodsExperimental autoimmune prostatitis (EAP) model, a well‐described and validated animal model of CP/CPPS, was used in our study. After treatment with CXCL10, the severity of EAP and Th1 cell proportion were respectively measured by HE stains, immunohistochemistry, and flow cytometry. Then, the protein expression of the PI3K/AKT pathway in CXCL10/CXCR3‐regulated Th1 cell differentiation and migration was evaluated by western blotting. Additionally, by the CXCR3 antagonist AMG487 and the PI3K inhibitor LY294002 applications, the effects of CXCL10/CXCR3 through PI3K/AKT pathway on the Th1 cell differentiation and migration were further assessed.ResultsThe EAP model was successfully built. CXCL10 increased the proportion of Th1 cells in EAP mice, accompanied by upregulation of the PI3K/AKT pathway. Additionally, the PI3K/AKT pathway was found to be involved in CXCL10/CXCR3 axis‐mediated Th1 cell differentiation and migration.ConclusionsOur investigations indicate that the CXCL10/CXCR3 axis regulates Th1 cell differentiation and migration in EAP through the PI3K/AKT pathway, which provides a new perspective on the immunological mechanisms of CP/CPPS.
Subject
Urology,Endocrinology,Reproductive Medicine,Endocrinology, Diabetes and Metabolism