Affiliation:
1. Shanxi Key Laboratory of Plant Macromolecules Stress Response TaiYuan P.R. China
2. School of Life Sciences Shanxi Normal University TaiYuan P.R. China
3. Department of New Biology DGIST Daegu Republic of Korea
Abstract
Abstract
The Ultraviolet Resistance Locus 8 (UVR8) in plants recognizes ultraviolet‐B (UV‐B) light and plays a crucial role in regulating plant growth through a series of signal transduction events. However, the UVR8 in monocotyledon crops has not yet been systematically analysed.
We identified BdUVR8 (BRADI_3g45740) from the genome of Brachypodium distachyon, a relative of wheat, by analysing the phylogenetic tree, the gene expression pattern, detecting accumulation of UV‐B response metabolites, and checking for phenotype recovery.
The BdUVR8 protein sequence is similar to the known UVR8 of other species. The phylogenetic tree of UVR8 shows clear divergence between dicotyledons and monocotyledons. Expression analysis revealed that UV‐B downregulates BdUVR8 by 70% and upregulates the chalcone synthase (BdCHS) gene 3.4‐fold in B. distachyon. The pCAMBIA1300::BdUVR8‐mCherry construct introduced into Arabidopsis uvr8 mutants showed that the BdUVR8 protein is localized in the cytoplasm and translocates into the nucleus in response to UV‐B irradiation. The introduction of BdUVR8 into uvr8 rescued hypocotyl elongation caused by UV‐B and restored expression of HY5, Chalcone synthase, and Flavanone 3‐hydroxylase, as well as accumulation of total flavonoids.
Together, our results show that BdUVR8 is a photoreceptor that perceives UV‐B in B. distachyon.
Funder
National Natural Science Foundation of China
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,General Medicine
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献