Genetic linkage map of Lolium multiflorum Lam. constructed from a BC1 population derived from an interspecific hybridization, L. multiflorum × Lolium temulentum L. × L. temulentum

Author:

Guan Xuanli12,Hirata Mariko3,Ding Chenglong4,Xu Nengxiang4,Yuyama Nana3,Tan Lubin125,Fu Yongcai12,Wang Jianping5,Cai Hongwei123

Affiliation:

1. Department of Plant Genetics and Breeding College of Agronomy and Biotechnology China Agricultural University Beijing China

2. Beijing Key Laboratory of Crop Genetic Improvement and Genome Ministry of Agriculture Beijing China

3. Forage Crop Research Institute Japan Grassland Agricultural and Forage Seed Association Nasushiobara Tochigi Japan

4. Institute of Animal Science Jiangsu Academy of Agricultural Sciences Nanjing China

5. Agronomy Department University of Florida Gainesville Florida USA

Abstract

AbstractSimple sequence repeat (SSR) markers and conserved‐intron scanning primer (CISP) markers developed from cereal expressed sequence tag sequences were successfully used to construct a linkage map for Lolium multiflorum Lam. using a L. multiflorum × Lolium temulentum L. × L. temulentum BC1 population consisting of 88 individuals. The genetic linkage map included 108 SSR and 84 CISP markers that covered 488.8 centimorgan (cM) in seven linkage groups, ranging in length from 42.2 to 98.9 cM. The average density of markers was 2.5 cM. Syntenic relationships between Lolium and rice (Oryza sativa L.) based on the positions of the CISP markers largely agree with the previously reported relationships. The map constructed in the present study will be useful in developing a denser linkage map as well as for analysis of quantitative trait loci for important agronomic traits in Lolium species.

Publisher

Wiley

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