Implications of CD45RA and CD45RO T cell subsets in patients of chronic rhinosinusitis with nasal polyposis infected with Aspergillus flavus

Author:

Rai Gargi1,Das Shukla1ORCID,Ansari Mohammad Ahmad1,Singh Praveen Kumar1,Dar Sajad Ahmad2ORCID,Gupta Neelima3,Sharma Sonal4,Ramachandran Vishnampettai Ganapathysubramanian1,Jain Charu1

Affiliation:

1. Department of Microbiology University College of Medical Sciences (University of Delhi) and Guru Teg Bahadur Hospital Delhi India

2. Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences Jazan University Jazan Saudi Arabia

3. Department of Otorhinolaryngology University College of Medical Sciences (University of Delhi) and Guru Teg Bahadur Hospital Delhi India

4. Department of Pathology University College of Medical Sciences (University of Delhi) and Guru Teg Bahadur Hospital Delhi India

Abstract

AbstractT cell subsets (CD4 and CD8) play a prominent role in the development of chronic rhinosinusitis with nasal polyposis (CRSwNP). Colonization with Aspergillus flavus is recognized as a trigger for the growth of nasal polyps. The fungal proteins initiate the recruitment of T cells into the nasal mucosa, which contributes to the progression of nasal polyps. The study included 50 cases of CRSwNP and 50 healthy controls. Biopsies were subjected to KOH and culture for mycological investigation. We examined the changes in T helper (CD4+) and T cytotoxic (CD8+) in total T cells (CD3+) and expression of naive (CD45RA) and memory (CD45RO) cell markers in T cell subsets in peripheral blood mononuclear cells (PBMCs) challenged by A. flavus antigens in cases before and after treatment and in healthy controls by flow cytometry. Predominantly, A. flavus (86%) identified in nasal polyp biopsies of patients. An increased percentage of CD3+CD4+ T cells observed after A. flavus stimulation in patients when compared with healthy controls. The expression of CD4+CD45RA+ cells was significantly (P < .05) reduced in patients and increased CD4+CD45RO+ was observed upon stimulation with A. flavus in patients when compared with healthy control. Continuous exposure to inhaled fungal spores may induce aberrant immune responses to A. flavus spores, causing an allergic immunological reaction with high CD4+T cell responses, resulting in an unfavourable outcome. Elevated CD4+CD45RO+ T cells may transform the pathogenic response and highlight the chances of A. flavus reactive T cells involvement in prompting inflammation in CRSwNP.

Funder

Indian Council of Medical Research

Publisher

Wiley

Subject

Immunology,General Medicine

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