Affiliation:
1. Laboratório de Biologia Molecular de Parasitas e Fungos Centro de Parasitologia e Micologia, Instituto Adolfo Lutz Sao Paulo Brazil
2. Programa de Pós‐graduação em Ciências da Coordenadoria de Controle de Doenças da Secretaria de Estado da Saúde de São Paulo Sao Paulo Brazil
3. Nucleo de Microscopia Eletrônica, Instituto Adolfo Lutz Sao Paulo Brazil
Abstract
AbstractThis retrospective cohort study analysed extracellular vesicles (EVs) and microRNAs (miRNAs) excreted in canine sera from dogs with canine visceral leishmaniasis (CanVL). A total of 56 canine sera were divided into Group I (28, from healthy dogs) and Group II (28, from the same dogs, but already with CanVL). CanVL was determined by clinical and laboratory diagnoses. Canine sera were ultra‐centrifuged to recover EVs (Can‐EVs). Analyses by transmission electron microscopy, nanoparticle tracking analysis (NTA), sodium dodecyl sulfate‐poli‐acrylammide gel eletroforesis (SDS‐PAGE) and, Immunoblot confirmed the presence of (i) microvesicles/exosomes and (ii) the tetraspanins CD63 and CD9. EVs secreted by Leishmania (Leishmania) infantum‐EVs were reactive against sera from dogs with CanVL (performed by ELISA and Immunoblot). NTA analyses exhibited that concentrations of Can‐EVs from dogs with CanVL (7.78 × 1010 Can‐EVs/mL) were higher (p < .0001) than the non‐infected dogs (mean: 1.47 × 1010 Can‐EVs/mL). These results suggested that concentrations of Can‐EVs were able to distinguish dogs with CanVL from healthy dogs. The relative expressions of 11 miRNAs species (miR‐21‐5p, miR‐146a‐5p, miR‐125b‐5p, miR‐144‐3p, miR‐194‐5p, miR‐346, miR‐29c‐3p, miR‐155‐5p, miR‐24‐3p, miR‐181a‐5p, and miR‐9‐5p) were estimated in purified miRNAs of 30 canine sera. Dogs with CanVL up‐expressed miR‐21‐5p and miR‐146a‐5p when compared with healthy dogs. The other miRNA species were poorly or not expressed in canine sera. In conclusion, this study suggests that CanVL induces changes in size and concentration of Can‐EVs, as well as, the up‐expression of miR‐21‐5p and miR‐146a‐5p in infected dogs.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Fundação de Amparo à Pesquisa do Estado de São Paulo
Reference72 articles.
1. WHO‐World Health Organization.Leishmaniasis.2022. Accessed December 10 2022.https://www.who.int/health-topics/leishmaniasis#tab=tab.
2. Leishmaniases of the New World: current concepts and implications for future research
3. Leishmaniasis Worldwide and Global Estimates of Its Incidence
4. PAHO–Pan American Health Organization.Visceral leishmaniasis.2022. Accessed December 10 2022.https://www.paho.org/en/topics/leishmaniasis/visceral-leishmaniasis.
5. MSB‐Ministerio da Saude do Brasil.Leishmaniose visceral. Distribuição da leishmaniose visceral no Brasil e no Mundo (Visceral leishmaniasis distribution in Brazil and worldwide).2022. Accessed December 10 2022. Site in Portuguese.https://www.gov.br/saude/pt-br/assuntos/saude-de-a-a-z/l/leishmaniose-visceral-1.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献