Putative epithelial–mesenchymal transitions during salamander limb regeneration: Current perspectives and future investigations

Author:

Kim Ryan T.1ORCID,Whited Jessica L.123ORCID

Affiliation:

1. Department of Stem Cell and Regenerative Biology Harvard University Cambridge Massachusetts USA

2. Broad Institute of Harvard and MIT Cambridge Massachusetts USA

3. Harvard Stem Cell Institute Harvard University Cambridge Massachusetts USA

Abstract

AbstractPrevious studies have implicated epithelial–mesenchymal transition (EMT) in salamander limb regeneration. In this review, we describe putative roles for EMT during each stage of limb regeneration in axolotls and other salamanders. We hypothesize that EMT and EMT‐like gene expression programs may regulate three main cellular processes during limb regeneration: (1) keratinocyte migration during wound closure; (2) transient invasion of the stump by epithelial cells undergoing EMT; and (3) use of EMT‐like programs by non‐epithelial blastemal progenitor cells to escape the confines of their niches. Finally, we propose nontraditional roles for EMT during limb regeneration that warrant further investigation, including alternative EMT regulators, stem cell activation, and fibrosis induced by aberrant EMT.

Funder

National Science Foundation

National Institute of Child Health and Human Development

Publisher

Wiley

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