1. Institute of Veterinary Medicine Federal University of Pará Castanhal Brazil
2. Laboratory of Semen Biotechnology and Andrology, School of Veterinary Medicine and Animal Science University of São Paulo Pirassununga Brazil
3. Vitrogen ‐ YVF Biotech Cravinhos Brazil
4. Department of Animal Nutrition and Production, College of Veterinary Medicine and Animal Science University of São Paulo Pirassununga Brazil
5. Laboratory of Biotechnology and Animal Reproduction, Embrapa Southeast Livestock (CPPSE/Embrapa) Brazilian Agricultural Research Corporation São Carlos Brazil