Affiliation:
1. Graduate School of Advanced Technology and Science Tokushima University Tokushima Japan
2. Graduate School of Agriculture Kyoto University Kyoto Japan
3. Division of Evolutionary Developmental Biology National Institute for Basic Biology Okazaki Japan
4. Graduate School of Sciences and Technology for Innovation Tokushima University Tokushima Japan
5. Bio‐Innovation Research Center Tokushima University Tokushima Japan
6. Technical Support Department Tokushima University Tokushima Japan
Abstract
AbstractThe acquisition of wings was a key event in insect evolution. As hemimetabolous insects were the first group to acquire functional wings, establishing the mechanisms of wing formation in this group could provide useful insights into their evolution. In this study, we aimed to elucidate the expression and function of the gene scalloped (sd), which is involved in wing formation in Drosophila melanogaster, and in Gryllus bimaculatus mainly during postembryonic development. Expression analysis showed that sd is expressed in the tergal edge, legs, antennae, labrum, and cerci during embryogenesis and in the distal margin of the wing pads from at least the sixth instar in the mid to late stages. Because sd knockout caused early lethality, nymphal RNA interference experiments were performed. Malformations were observed in the wings, ovipositor, and antennae. By analyzing the effects on wing morphology, it was revealed that sd is mainly involved in the formation of the margin, possibly through the regulation of cell proliferation. In conclusion, sd might regulate the local growth of wing pads and influence wing margin morphology in Gryllus.
Funder
Ministry of Education, Culture, Sports, Science and Technology
Subject
Cell Biology,Developmental Biology
Cited by
1 articles.
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