Livin is protective in UVB‐induced skin photodamage by regulating keratinocyte activation and inflammatory responses

Abstract

AbstractUVB radiation can lead to skin photodamage, which might arise from keratinocyte (KC) activation. Nuclear factor kappa B (NF‐κB) assumes an essential function in the context of UVB‐triggered skin photodamage. Initiating the NF‐κB cascade leads to the release of inflammatory factors from KCs. Livin can modulate both KC activation and function, yet it remains uncertain whether and how Livin regulates KC activation induced by UVB. To explore the involvement of Livin in UVB‐triggered skin photodamage and its impact on skin damage through NF‐κB activation. Immunofluorescence staining was used to analyse the expression of Livin in individuals with skin photodamage and in mice treated with UVB radiation. KC‐specific Livin knockout (LivinΔKC) mice and HaCaT cells with Livin knockdown were employed to examine the function of Livin in regulating KC activation induced by UVB radiation. Additionally, the impact of Livin on the NF‐κB cascade during KC activation was confirmed via western blot analysis. In patients with skin photodamage, UVB‐treated mice and HaCaT cells, Livin expression was reduced in KCs. LivinΔKC mice displayed heightened sensitivity to UVB radiation, resulting in more pronounced skin damage and inflammatory responses compared to the control Livinfl/fl mice. Following UVB exposure, both LivinΔKC mice and Livin‐knockdown HaCaT cells released elevated levels of cytokines compared to their respective controls. Moreover, the UVB‐induced activation of NF‐κB in HaCaT cells was significantly enhanced following Livin knockdown. Our findings propose that Livin within KCs could contribute to reducing UVB‐induced skin photodamage by regulating the NF‐κB pathway.