Baccharis dracunculifolia DC (Asteraceae) selectively modulates the effector functions of human neutrophils

Author:

Figueiredo-Rinhel Andréa S G1,de Melo Lamartine L12,Bortot Leandro O1,Santos Everton O L12,Andrade Micássio F12,Azzolini Ana Elisa C S1,Kabeya Luciana M1,Caliri Antonio1,Bastos Jairo K3,Lucisano-Valim Yara Maria1ORCID

Affiliation:

1. Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto da Universidade de São Paulo, Ribeirão Preto, SP, Brazil

2. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirão Preto, SP, Brazil

3. Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto da Universidade de São Paulo, Ribeirão Preto, SP, Brazil

Abstract

Abstract Objectives To examine whether the hydroalcoholic extract from Baccharis dracunculifolia leaves (BdE) modulates the human neutrophil oxidative metabolism, degranulation, phagocytosis and microbial killing capacity. Methods In-vitro assays based on chemiluminescence, spectrophotometry, flow cytometry and polarimetry were used, as well as docking calculations. Key findings At concentrations that effectively suppressed the neutrophil oxidative metabolism elicited by soluble and particulate stimuli (<10 μg/ml), without clear signs of cytotoxicity, BdE (1) inhibited NADPH oxidase and myeloperoxidase activity; (2) scavenged H2O2 and HOCl; (3) weakly inhibited phagocytosis; and (4) did not affect neutrophil degranulation and microbial killing capacity, the expression levels of TLR2, TLR4, FcγRIIa, FcγRIIIb and CR3 and the activity of elastase and lysozyme. Caffeic acid, one of the major B. dracunculifolia secondary metabolites, did not inhibit phagocytosis but interfered in the myeloperoxidase–H2O2–HOCl system by scavenging H2O2 and HOCl, and interacting with the catalytic residues His-95, Arg-239 and Gln-91. Conclusions BdE selectively modulates the effector functions of human neutrophils, inhibits the activity of key enzymes and scavenges physiological oxidant species. Caffeic acid contributes to lower the levels of oxidant species. Our findings help to unravel the mechanisms by which these natural products exert immunomodulatory action towards neutrophils.

Funder

Brazilian funding agencies São Paulo Research Foundation

National Council for Scientific and Technological Development

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

Reference54 articles.

1. Human neutrophils in auto-immunity;Thieblemont;Semin Immunol,2016

2. Anti-inflammatory activity of natural products;Azab;Molecules,2016

3. Marine natural product inhibitors of neutrophil-associated inflammation;Chen;Mar Drugs,2016

4. Bioassay guided fractionation - an emerging technique influence the isolation, identification and characterization of lead phytomolecules;Malviya;IJHP,2017

5. Effect of Brazilian green propolis on the production of reactive oxygen species by stimulated neutrophils;Simões;J Ethnopharmacol,2004

Cited by 10 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3