Affiliation:
1. Biophotonics Applied to Health Science Nove de Julho University Sao Paulo SP Brazil
2. Cardiology Division, Department of Medicine Federal University of Sao Paulo São Paulo SP Brazil
3. Base Hospital Foundation Medicine School of São José do Rio Preto Sao Paulo SP Brazil
Abstract
AbstractThis study investigated the influence of red light‐emitting diodes (LED, 630 nm) on different irradiation parameters and the number of applications on mesenchymal stem cells derived from adipose tissue (AdMSCs) metabolism and paracrine factors. The AdMSCs were irradiated with a LEDbox device (output power: 2452.5 mW; laser beam: 163.5 cm2; irradiance: 15 mW cm−2) using radiant exposures of 0.5, 2, and 4 J cm−2, respectively. AdMSCs were irradiated once or every 48 h up to three irradiations. All molecular analyses were performed 24 h after the last irradiation. LED did not induce changes in cell count, DNA damage, and oxidative stress. A significant repercussion of the LED has been noticed after three irradiations with 4 J cm−2. AdMSCs had higher levels of IL‐6, IGF‐1, and NOx index. A higher ATP content and MMT/Resazurin assay were identified in AdMSCs irradiated three times with 4 J cm−2. Mitochondrial basal respiration, maximal respiration and proton leak under metabolic stress were reduced by 0.5 and 2 J cm−2 irradiations. These data showed that three LED irradiations with 4 J cm−2 may be a suitable parameter for future AdMSCs therapy because of its improved metabolic activity, ATP content, and IL‐6, IGF‐1, and nitric oxide secretion.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa do Estado de São Paulo
Subject
Physical and Theoretical Chemistry,General Medicine,Biochemistry