A novel Pik allele confers extended resistance to rice blast

Author:

Qi Zhongqiang12ORCID,Meng Xiuli345,Xu Ming6,Du Yan1,Yu Junjie1,Song Tianqiao1,Pan Xiayan1,Zhang Rongsheng1,Cao Huijuan1,Yu Mina1,Telebanco‐Yanoria Mary Jeanie4,Lu Guodong3,Zhou Bo24,Liu Yongfeng12

Affiliation:

1. Institute of Plant Protection Jiangsu Academy of Agricultural Science Nanjing China

2. IRRI‐JAAS Joint Laboratory Jiangsu Academy of Agricultural Science Nanjing China

3. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection Fujian Agriculture and Forestry University (FAFU) Fuzhou China

4. Genetics and Biotechnology Division International Rice Research Institute, College Los Banos Laguna Philippines

5. Coconut Research Institute Chinese Academy of Tropical Agricultural Sciences Wenchang China

6. High‐throughput Genotyping Shared Laboratory Seed Administration Department of Jiangsu Province Nanjing China

Abstract

AbstractIn the ongoing arms race between rice and Magnaporthe oryzae, the pathogen employs effectors to evade the immune response, while the host develops resistance genes to recognise these effectors and confer resistance. In this study, we identified a novel Pik allele, Pik‐W25, from wild rice WR25 through bulked‐segregant analysis, creating the Pik‐W25 NIL (Near‐isogenic Lines) named G9. Pik‐W25 conferred resistance to isolates expressing AvrPik‐C/D/E alleles. CRISPR‐Cas9 editing was used to generate transgenic lines with a loss of function in Pik‐W25‐1 and Pik‐W25‐2, resulting in loss of resistance in G9 to isolates expressing the three alleles, confirming that Pik‐W25‐induced immunity required both Pik‐W25‐1 and Pik‐W25‐2. Yeast two‐hybrid (Y2H) and split luciferase complementation assays showed interactions between Pik‐W25‐1 and the three alleles, while Pik‐W25‐2 could not interact with AvrPik‐C, ‐D, and E alleles with Y2H assay, indicating Pik‐W25‐1 acts as an adaptor and Pik‐W25‐2 transduces the signal to trigger resistance. The Pik‐W25 NIL exhibited enhanced field resistance to leaf and panicle blast without significant changes in morphology or development compared to the parent variety CO39, suggesting its potential for resistance breeding. These findings advance our knowledge of rice blast resistance mechanisms and offer valuable resources for effective and sustainable control strategies.

Publisher

Wiley

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