Affiliation:
1. Department of Gastroenterology City Children's Hospital Ho Chi Minh City Vietnam
2. Faculty of Medicine Université Libre de Bruxelles Brussels Belgium
3. Department of Microbiology and Parasitology University of Medicine and Pharmacy at Ho Chi Minh City Ho Chi Minh City Vietnam
4. Department of Genetics University of Science – Vietnam National University Ho Chi Minh City Ho Chi Minh City Vietnam
5. Department of Gastroenterology Children's Hospital 2 Ho Chi Minh City Vietnam
6. Department of Biostatistics and Informatics Pham Ngoc Thach University of Medicine Ho Chi Minh City Vietnam
7. Faculty of Public Health Pham Ngoc Thach University of Medicine Ho Chi Minh City Vietnam
8. Department of Surgery City Children's Hospital Ho Chi Minh City Vietnam
9. Institut de recherche expérimentale et clinique, Pôle d'épidémiologie et Biostatistique, Université catholique de Louvain Brussels Belgium
10. Department of Gastroenterology Hôpital Universitaire des Enfants Reine Fabiola Brussels Belgium
Abstract
AbstractBackgroundAntibiotic resistance of Helicobacter pylori (H. pylori) is increasing worldwide, with geographical variations, impacting the treatment outcomes. This study assessed the antibiotic resistance patterns of H. pylori in Vietnamese children.Materials and MethodsSymptomatic children undergoing gastroduodenoscopy at two tertiary Children's Hospitals in Ho Chi Minh City were recruited. Antral and corpus biopsies were obtained and cultured separately. Susceptibility to amoxicillin (AMO), clarithromycin (CLA), metronidazole (MET), levofloxacin (LEV), and tetracycline (TET) was determined using E‐test. Polymerase chain reaction was performed on another antral biopsy to detect the urease gene, cytotoxin‐associated gene A (cagA), vacuolating cytotoxin A (vacA) genotypes, and 23S rRNA mutations conferring CLA resistance.ResultsAmong 123 enrolled children, a high primary resistance rate was found for CLA (68.5%, 61/89), followed by LEV (55.1%), MET (31.5%), AMO (25.8%), and TET (1.1%). Secondary resistance rates were 82.1% (7/28), 71.4%, 53.6%, and 3.6% for CLA, LEV, MET, and TET, respectively. Multidrug resistance was frequent (67.7%), with common patterns including CLA + LEV (20.3%) and CLA + MTZ + LEV (15.2%). Heteroresistance was detected in eight children (6.5%). The A2143G mutation was detected in 97.5% (119/122) of children. 86.1% of children had positive cagA strains and 27.9% had multiple vacA genotypes. No factor was significantly associated with antibiotic resistance.ConclusionsThe alarming rate of antibiotic resistance for H. pylori, especially for CLA, with emerging multi‐ and hetero‐resistant strains, pose a major treatment challenge that precludes CLA use as empirical therapy. Biopsies from both antrum and corpus can improve H. pylori culture, allowing tailored treatment based on antimicrobial susceptibility.
Subject
Infectious Diseases,Gastroenterology,General Medicine
Cited by
2 articles.
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