Protein stability governed by α1–2 helices in Pvr4 is essential for localization and cell death

Author:

Lee Hye‐Young1,Choi Jeen12,Kang Minji12,Lee Joo Hyun1,Kim Myung‐Shin13,Choi Doil12ORCID

Affiliation:

1. Plant Immunity Research Center Seoul National University Seoul 08826 Republic of Korea

2. Horticultural Science and Biotechnology Program, Department of Agriculture, Forestry and Bioresources Plant Genomics and Breeding Institute, Seoul National University Seoul 08826 Republic of Korea

3. Department of Biosciences and Bioinformatics Myongji University Yongin 17058 Republic of Korea

Abstract

SUMMARYPlant nucleotide‐binding domain leucine‐rich‐repeat receptor (NLR) confers disease resistance to various pathogens by recognizing effectors derived from the pathogen. Previous studies have shown that overexpression of the CC domain in several NLRs triggers cell death, implying that the CC domain plays an important role as a signaling module. However, how CC domain transduces immune signals remains largely unknown. A Potyvirus‐resistant NLR protein, Pvr4, possesses a CC domain (CCPvr4) that induces cell death upon transient overexpression in Nicotiana benthamiana. In this study, loss‐of‐function mutants were generated by error‐prone PCR‐based random mutagenesis to understand the molecular mechanisms underlying CCPvr4‐mediated cell death. Cell biology and biochemical studies revealed that M16 and Q52 in the α1 and α2 helices, respectively, are crucial for protein stability, and mutation of these residues disrupts localization to the plasma membrane and oligomerization activity. The increase of the protein stability of these mutants by tagging a green fluorescent protein (GFP) variant led to restoration of cell death‐inducing activity and plasma membrane localization. Another mutant, I7E in the very N‐terminal region, lost cell death‐inducing activity by weakening the interaction with plasma membrane H+‐ATPase compared to CCPvr4, although the protein remained in the plasma membrane. Moreover, most of the mutated residues are on the outer surface of the funnel shape in the predicted pentameric CCPvr4, implying that the disordered N‐terminal region plays a crucial role in association with PMA as well as targeting to the plasma membrane. This work could provide insights into the molecular mechanisms of cell death induced by NLR immune receptors.

Funder

National Research Foundation

Publisher

Wiley

Subject

Cell Biology,Plant Science,Genetics

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