Affiliation:
1. School of Dentistry, Health Science Institute Paulista University São Paulo Brazil
2. CEEpiRG, Program in Environmental and Experimental Pathology Paulista University – UNIP São Paulo Brazil
3. Department of Chemistry and Biochemistry, Lab. of Bioassays and Cellular Dynamics, Institute of Biosciences UNESP – São Paulo State University Botucatu Brazil
4. Department of Clinical Dentistry Federal University of Espírito Santo Vitória Brazil
5. Division of Periodontics, Institute of Science and Technology UNESP – São Paulo State University São Paulo Brazil
Abstract
AbstractBackgroundHere, we evaluated whether the histone lysine demethylase 5B (JARID1B), is involved in osteogenic phenotype commitment of periodontal ligament cells (PDLCs), by considering their heterogeneity for osteoblast differentiation.Materials and MethodsEpigenetic, transcriptional, and protein levels of a gene set, involved in the osteogenesis, were investigated by performing genome‐wide DNA (hydroxy)methylation, mRNA expression, and western blotting analysis at basal (without osteogenic induction), and at the 3rd and 10th days of osteogenic stimulus, in vitro, using PDLCs with low (l) and high (h) osteogenic potential as biological models.Resultsh‐PDLCs showed reduced levels of JARID1B, compared to l‐PDLCs, with significant inversely proportional correlations between RUNX2 and RUNX2/p57. Epigenetically, a significant reduction in the global H3K4me3 content was observed only in h‐PDLCs. Immunoblotting data reveal a significant reduction in the global H3K4me3 content, at 3 days of induction only in h‐PDLCs, while an increase in the global H3K4me3 content was observed at 10 days for both PDLCs. Additionally, positive correlations were found between global H3K4me3 levels and JARID1B gene expression.ConclusionsAltogether, our results show the crucial role of JARID1B in repressing PDLCs osteogenic phenotype and this claims to pre‐clinical protocols proposing JARID1B as a potential therapeutic target.
Subject
General Dentistry,Otorhinolaryngology