Affiliation:
1. Center of Stomatology, Tongji Hospital, Tongji Medical College Huazhong University of Science and Technology Wuhan China
2. Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration Wuhan China
Abstract
AbstractObjectiveThis study aimed to investigate the effect of proanthocyanidin (PA) on osteogenesis mediated by periodontal ligament stem cells (PDLSCs) and endogenous alveolar bone regeneration.BackgroundLeveraging the osteogenic potential of resident stem cells is a promising strategy for alveolar bone regeneration. PA has been reported to be effective in osteogenesis. However, the effect and mechanism of PA on the osteogenic differentiation of PDLSCs remain elusive.MethodsHuman PDLSCs were treated with various doses of PA to assess the cell proliferation using Cell Counting Kit‐8. The osteogenic differentiation ability was detected by qRT‐PCR analysis, western blot analysis, Alizarin red S staining, and Alkaline Phosphatase staining. The level of autophagy was evaluated by confocal laser scanning microscopy, transmission electron microscopy, and western blot analysis. RNA sequencing was utilized to screen the potential signaling pathway. The alveolar bone defect model of rats was created to observe endogenous bone regeneration.ResultsPA activated intracellular autophagy in PDLSCs, resulting in enhanced osteogenic differentiation. Moreover, this effect could be abolished by the autophagy inhibitor 3‐Methyladenine. Mechanistically, the PI3K/Akt/mTOR pathway was negatively correlated with PA‐mediated autophagy activation. Lastly, PA promoted the alveolar bone regeneration in vivo, and this effect was reversed when the autophagy process was blocked.ConclusionPA may activate autophagy by inhibiting PI3K/Akt/mTOR signaling pathway to promote the osteogenesis of PDLSCs and enhance endogenous alveolar bone regeneration.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Hubei Province