Directed evolution of an artificial bifunctional enzyme, γ-glutamyl kinase/γ-glutamyl phosphate reductase, for improved osmotic tolerance ofEscherichia colitransformants

Author:

Chen Mingqing,Cao Junwei,Zheng Congyi,Liu Qing

Publisher

Oxford University Press (OUP)

Subject

Genetics,Molecular Biology,Microbiology

Reference22 articles.

1. Csonka LN (1981) Proline over-production results in enhanced osmotolerance in Salmonella typhimurium. Mol Gen Genet 182: 82–86.

2. Csonka LN , Gelvin SB , Goodner BW , Orser CS , Siemieniak D & Slightom JL (1988) Nucleotide sequence of a mutation in the proB gene of Escherichia coli that confers proline overproduction and enhanced tolerance to osmotic stress. Gene 64: 199–205.

3. Dandekar AM & Uratsu SL (1988) A single base pair change in proline biosynthesis genes causes osmotic stress tolerance. J Bacteriol 170: 5943–5945.

4. Deutch AH , Rushlow KE & Smith CJ (1984) Analysis of the Escherichia coliproBA locus by DNA and protein sequencing. Nucleic Acids Res 12: 6337–6355.

5. Fiedler M & Skerra A (2001) proBA complementation of an auxotrophic E. coli strain improves plasmid stability and expression yield during fermented production of a recombinant antibody fragment. Gene 274: 111–118.

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