Using site-saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate-dependent enzymes
Author:
Affiliation:
1. Department of Chemistry and Chemical Biology; Indiana University-Purdue University Indianapolis; IN USA
Publisher
Wiley
Subject
Cell Biology,Molecular Biology,Biochemistry
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1111/febs.12459/fullpdf
Reference111 articles.
1. Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function;Miyazaki;J Mol Evol,1999
2. Distributions of enzyme residues yielding mutants with improved substrate specificities from two different directed evolution strategies;Paramesvaran;Prot Eng Des Sel,2009
3. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction;Leung;Technique,1989
4. Rapid evolution of a protein in vitro by DNA shuffling;Stemmer;Nature,1994
5. Optimising enzyme function by directed evolution;Dalby;Curr Opin Struct Biol,2003
Cited by 19 articles. 订阅此论文施引文献 订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
1. Mutant β-fructofuranosidase synthesizing blastose [β-d-Fruf-(2→6)-d-Glcp];Enzyme and Microbial Technology;2024-10
2. Engineering a PbrR-Based Biosensor for Cell-Free Detection of Lead at the Legal Limit;ACS Synthetic Biology;2024-09-10
3. Engineering the 2-Oxoglutarate Dehydrogenase Complex to Understand Catalysis and Alter Substrate Recognition;Reactions;2022-02-01
4. Continuous enzymatic stirred tank reactor cascade with unconventional medium yielding high concentrations of (S)-2-hydroxyphenyl propanone and its derivatives;Catalysis Science & Technology;2021
5. An Update on Developments in the Field of Thiamin Diphosphate-Dependent Enzymes;Comprehensive Natural Products III;2020
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3