The double-antigen ELISA concept for early detection of Erns-specific classical swine fever virus antibodies and application as an accompanying test for differentiation of infected from marker vaccinated animals

Author:

Meyer D.1,Fritsche S.2,Luo Y.3,Engemann C.2,Blome S.4ORCID,Beyerbach M.5,Chang C.-Y.6,Qiu H.-J.3,Becher P.1,Postel A.1ORCID

Affiliation:

1. EU and OIE Reference Laboratory for Classical Swine Fever; Institute of Virology; Department of Infectious Diseases; University of Veterinary Medicine Hannover; Hannover Germany

2. QIAGEN Leipzig GmbH; Leipzig Germany

3. State Key Laboratory of Veterinary Biotechnology; Harbin Veterinary Research Institute (HVRI); Chinese Academy of Agricultural Sciences; Harbin China

4. Institute of Diagnostic Virology; Friedrich-Loeffler-Institut; Greifswald Germany

5. Institute for Biometry; Epidemiology and Information Processing; University of Veterinary Medicine Hannover; Hannover Germany

6. Animal Health Research Institute; Council of Agriculture; Executive Yuan; Tamsui District New Taipei City Taiwan

Funder

Zentrales Innovationsprogramm Mittelstand

Bundesministerium für Wirtschaft und Energie

Publisher

Wiley

Subject

General Veterinary,General Immunology and Microbiology,General Medicine

Reference22 articles.

1. Two newly developed E(rns)-based ELISAs allow the differentiation of Classical Swine Fever virus-infected from marker-vaccinated animals and the discrimination of pestivirus antibodies;Aebischer;Veterinary Microbiology,2013

2. Anonymous 2007 EU Diagnostic Manual for Classical swine fever (CSF) Diagnosis: Technical Part (Third Draft June 2007) http://www.tiho-hannover.de/fileadmin/user_upload/tiho_hannover/kliniken_institute/21_virologie/eurl/Technical_Annex_Draft_2007.pdf

3. Anonymous 2016 Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2015, Chapter 1.1.6: Principles and methods of validation of diagnostic assays for infectious diseases http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/1.01.06_VALIDATION.pdf

4. Efficacy of marker vaccine candidate CP7_E2alf against challenge with classical swine fever virus isolates of different genotypes;Blome;Veterinary Microbiology,2014

5. Molecular specificity of the antibody responses of cattle naturally and experimentally infected with cytopathic and noncytopathic bovine viral diarrhea virus biotypes;Donis;American Journal of Veterinary Research,1987

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