The combination of breast cancer PDO and mini‐PDX platform for drug screening and individualized treatment

Author:

Cui Yuxin123,Ran Ran123,Da Yanyan14,Zhang Huiwen1,Jiang Meng123,Qi Xin123,Zhang Wei5,Niu Ligang5,Zhou Yuhui5,Zhou Can5,Tang Xiaojiang5,Wang Ke5,Yan Yu5,Ren Yu5,Dong Danfeng123,Zhou Yan123,Wang Hui123,Gong Jin123,Hu Fang123,Zhao Shidi123,Zhang Huimin5ORCID,Zhang Chengsheng124,Yang Jin123ORCID

Affiliation:

1. Precision Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi People's Republic of China

2. Cancer Center, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi People's Republic of China

3. Department of Medical Oncology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi People's Republic of China

4. Center for Molecular Diagnosis and Precision Medicine The First Affiliated Hospital, Jiangxi Medical College, Nanchang University Nanchang Jiangxi China

5. Department of Breast Surgery The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi People's Republic of China

Abstract

AbstractThe majority of advanced breast cancers exhibit strong aggressiveness, heterogeneity, and drug resistance, and currently, the lack of effective treatment strategies is one of the main challenges that cancer research must face. Therefore, developing a feasible preclinical model to explore tailored treatments for refractory breast cancer is urgently needed. We established organoid biobanks from 17 patients with breast cancer and characterized them by immunohistochemistry (IHC) and next generation sequencing (NGS). In addition, we in the first combination of patient‐derived organoids (PDOs) with mini‐patient‐derived xenografts (Mini‐PDXs) for the rapid and precise screening of drug sensitivity. We confirmed that breast cancer organoids are a high‐fidelity three‐dimension (3D) model in vitro that recapitulates the original tumour's histological and genetic features. In addition, for a heavily pretreated patient with advanced drug‐resistant breast cancer, we combined PDO and Mini‐PDX models to identify potentially effective combinations of therapeutic agents for this patient who were alpelisib + fulvestrant. In the drug sensitivity experiment of organoids, we observed changes in the PI3K/AKT/mTOR signalling axis and oestrogen receptor (ER) protein expression levels, which further verified the reliability of the screening results. Our study demonstrates that the PDO combined with mini‐PDX model offers a rapid and precise drug screening platform that holds promise for personalized medicine, improving patient outcomes and addressing the urgent need for effective therapies in advanced breast cancer.

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3