Affiliation:
1. Medical Research Center, The Third People's Hospital of Chengdu The Affiliated Hospital of Southwest Jiaotong University Chengdu Sichuan China
2. Department of Cardiology The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu Chengdu China
3. Pharmacist‐In‐Charge Department of Pharmacy Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu Chengdu Sichuan China
Abstract
AbstractBackground and AimExosome‐like nanoparticles (ELNs) have emerged as crucial mediators of intercellular communication, evaluated as potential bioactive nutraceutical biomolecules. We hypothesized that oral ELNs have some therapeutic effect on irritable bowel syndrome (IBS).MethodsIn our study, ELNs from tea (Camellia sinensis) leaves were extracted by differential centrifugation. We investigated the role of ELNs by assessing visceral hypersensitivity, body weight, bowel habits, tight junctions, and corticotropin‐releasing hormone (CRH) in rats subjected to water avoidance stress (WAS) to mimic IBS with and without ELNs (1 mg/kg per day) for 10 days.ResultsThe average diameter of ELNs from LCC, FD and MZ tea tree were 165 ± 107, 168 ± 94, and 168 ± 108 nm, the concentration of ELNs were 1.2 × 1013, 1 × 1013, and 1.5 × 1013 particles/mL, respectively. ELNs can be taken up by intestinal epithelial cells. In WAS rats, ELNs significantly restored weight, recovered tight junctions, decreased CRH, and CRH receptor 1 expression levels and inhibited abdominal hypersensitivity in comparison to positive control.ConclusionsOral tea‐derived ELN improves symptoms of IBS by potentially modulating the CRH pathway.
Funder
National Natural Science Foundation of China
Sichuan Province Science and Technology Support Program