Affiliation:
1. Department of Urology Sun Yat‐sen Memorial Hospital, Sun Yat‐sen University Guangzhou China
2. Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Sun Yat‐sen Memorial Hospital, Sun Yat‐sen University Guangzhou China
3. Guangdong Provincial Clinical Research Center for Urological Diseases Guangzhou China
4. Department of Hepatobiliary Surgery Sun Yat‐Sen Memorial Hospital, Sun Yat‐Sen University Guangzhou China
Abstract
AbstractCircular RNAs (circRNAs) play crucial roles in various biological processes, including prostate cancer (PCa). However, the precise roles and mechanism of circRNAs are complicated. Hence, we studied the function of a circRNA that might be involved in the progression of PCa. In this study, we found that circARHGEF28 was frequently downregulated in PCa tissues and cell lines. Furthermore, gain‐ and loss‐of function experiments in vitro showed that circARHGEF28 inhibited proliferation, migration, and invasion of PCa. Additionally, circARHGEF28 suppressed PCa progression in vivo. Bioinformatics analysis and RNA pull‐down and capture assay found that circARHGEF28 sponged miR‐671‐5p in PCa cells. Importantly, qRT‐PCR and dual luciferase assays found that Lectin galactoside‐binding soluble 3 binding protein (LGALS3BP) was downstream of miR‐671‐5p, and western blot analysis further confirmed that LGALS3BP negatively regulated the nuclear factor kappa‐B (NF‐κB) pathway. These results demonstrated that circARHGEF28 abolished the degradation of LGALS3BP by sponging miR‐671‐5p, thus blocking the activation of the NF‐κB pathway. Our findings revealed that circARHGEF28/miR‐671‐5p/LGALS3BP/NF‐κB may be an important axis that regulates PCa progression.
Funder
Guangdong Science and Technology Department
National Natural Science Foundation of China
Subject
Cancer Research,Oncology,General Medicine
Cited by
3 articles.
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