Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants

Author:

Muniz C.R.1,Freire F.C.O.1,Viana F.M.P.1,Cardoso J.E.1,Correia D.1,Jalink H.2,Kema G.H.J.3,Silva G.F.4,Guedes M.I.F5

Affiliation:

1. Laboratório de Fitopatologia; Embrapa Agroindústria Tropical; Fortaleza; Ceará; Brazil

2. Wageningen University Greenhouse Horticulture; 6700 AP; Wageningen; The Netherlands

3. Biointeractions and Plant Health; Plant Research International B.V.; 6700 AA; Wageningen; The Netherlands

4. Laboratório de Biologia Molecular; Embrapa Amazônia Ocidental; Manaus; Amazônia; Brazil

5. Centro de Ciência da Saúde; Universidade Estadual do Ceará; Fortaleza; Ceará; Brazil

Publisher

Wiley

Subject

Agronomy and Crop Science

Reference34 articles.

1. Botryosphaeria corticola sp. nov. on Quercus species, with notes and description of Botryosphaeria stevensii and its anamorph Diplodia mutila;Alves;Mycologia,2004

2. Morphological and molecular data reveal cryptic species in Lasiodiplodia theobromae;Alves;Fungal Diversity,2008

3. The solid phase attachment of fungal hyphae in an ELISA to screen for antifungal antibodies;Banks;Mycopathologia,1992

4. A rapid and sensitive assay of protein utilizing the principle of dye binding;Bradford;Analytical Biochemistry,1976

5. Efeito de práticas agronômicas na ocorrência da resinose do cajueiro;Cardoso;Fitopatologia Brasileira,1995

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