Comparative metabolic profiling of the mycelium and fermentation broth of Penicillium restrictum from Peucedanum praeruptorum rhizosphere

Author:

Wang Yuanyuan12,Liao Ranran12,Pan Haoyu23,Wang Xuejun2,Wan Xiaoting12,Han Bangxing12,Song Cheng2ORCID

Affiliation:

1. School of Pharmacy Anhui University of Chinese Medicine Hefei China

2. Anhui Dabieshan Academy of Traditional Chinese Medicine, Anhui Engineering Research Center for Eco‐agriculture of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering West Anhui University Luan China

3. School of Life Science Anhui Agricultural University Hefei China

Abstract

AbstractMicroorganisms in the rhizosphere, particularly arbuscular mycorrhiza, have a broad symbiotic relationship with their host plants. One of the major fungi isolated from the rhizosphere of Peucedanum praeruptorum is Penicillium restrictum. The relationship between the metabolites of P. restrictum and the root exudates of P. praeruptorum is being investigated. The accumulation of metabolites in the mycelium and fermentation broth of P. restrictum was analysed over different fermentation periods. Non‐targeted metabolomics was used to compare the differences in intracellular and extracellular metabolites over six periods. There were significant differences in the content and types of mycelial metabolites during the incubation. Marmesin, an important intermediate in the biosynthesis of coumarins, was found in the highest amount on the fourth day of incubation. The differential metabolites were screened to obtain 799 intracellular and 468 extracellular differential metabolites. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the highly enriched extracellular metabolic pathways were alanine, aspartate and glutamate metabolism, glyoxylate and dicarboxylate metabolism, and terpenoid backbone biosynthesis. In addition, the enrichment analysis associated with intracellular and extracellular ATP‐binding cassette transporter proteins revealed that some ATP‐binding cassette transporters may be involved in the transportation of certain amino acids and carbohydrates. Our results provide some theoretical basis for the regulatory mechanisms between the rhizosphere and the host plant and pave the way for the heterologous production of furanocoumarin.

Funder

National Key Research and Development Program of China

Publisher

Wiley

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