The secretome of Thermococcus barophilus in the presence of carbohydrates and the potential role of the TrmBL4 regulator

Author:

Batour Maria12,Laurent Sébastien1,Moalic Yann13ORCID,Chamieh Hala2,Taha Samir2,Jebbar Mohamed1ORCID

Affiliation:

1. Univ Brest, CNRS, Ifremer, Laboratoire de Biologie et d'Écologie des Écosystèmes marins profonds (BEEP), IUEM Plouzané France

2. Laboratory of Applied Biotechnology, Azm Center for Research in Biotechnology and Its Applications Lebanese University Tripoli Lebanon

3. LabISEN, Yncréa Ouest Brest France

Abstract

AbstractGlobal transcriptional regulators are crucial for supporting rapid adaptive responses in changing environments. In Thermococcales, the TrmB sugar‐sensing regulator family is well represented but knowledge of the functional role/s of each of its members is limited. In this study, we examined the link between TrmBL4 and the degree of protein secretion in different sugar environments in the hyperthermophilic Archaeon Thermococcus barophilus. Although the absence of TrmBL4 did not induce any growth defects, proteomics analysis revealed different secretomes depending on the sugar and/or genetic contexts. Notably, 33 secreted proteins present in the supernatant were differentially detected. Some of these proteins are involved in sugar assimilation and transport, such as the protein encoded by TERMP_01455 (cyclomaltodextrin glucanotransferase), whereas others have intracellular functions, such as the protein encoded by TERMP_01556 (pyruvate: ferredoxin oxidoreductase Δsubunit). Then, using reverse transcription quantitative polymerase chain reaction experiments, we observed effective transcription regulation by TrmBL4 of the genes encoding at least two ABC‐type transporters according to sugar availability.

Funder

ISblue

Publisher

Wiley

Subject

Agricultural and Biological Sciences (miscellaneous),Ecology, Evolution, Behavior and Systematics

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3