Affiliation:
1. Hebei Key Laboratory of Close‐to‐Nature Restoration Technology of Wetlands Hebei University Baoding People's Republic of China
2. Institute of Xiong'an New Area Hebei University Baoding People's Republic of China
3. School of Eco‐Environment Hebei University Baoding People's Republic of China
4. College of Life Science Hebei University Baoding People's Republic of China
Abstract
AbstractResolving the heavy metal resistance mechanisms of microbes is crucial for understanding the bioremediation of the ecological environment. In this study, a multiple heavy metal resistance bacterium, Pseudoxanthomonas spadix ZSY‐33 was isolated and characterized. The copper resistance mechanism was revealed by analysis of the physiological traits, copper distribution, and genomic and transcriptomic data of strain ZSY‐33 cultured with different concentrations of copper. The growth inhibition assay in basic medium showed that the growth of strain ZSY‐33 was inhibited in the presence of 0.5 mM copper. The production of extracellular polymeric substances increased at a lower concentration of copper and decreased at a higher concentration of copper. Integrative analysis of genomic and transcriptomic, the copper resistance mechanism in strain ZSY‐33 was elucidated. At a lower concentration of copper, the Cus and Cop systems were responsible for the homeostasis of intracellular copper. As the concentration of copper increased, multiple metabolism pathways, including the metabolism of sulfur, amino acids, and pro‐energy were cooperated with the Cus and Cop systems to deal with copper stress. These results indicated a flexible copper resistance mechanism in strain ZSY‐33, which may acquire from the long‐term interaction with the living environment.
Funder
China Postdoctoral Science Foundation
Subject
Agricultural and Biological Sciences (miscellaneous),Ecology, Evolution, Behavior and Systematics
Cited by
2 articles.
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