Don't put all your eggs in one basket: a cost‐effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array-Reference-Cited by-同舟云学术

Don't put all your eggs in one basket: a cost‐effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array

Published:2016-02-26 Issue:4 Volume:16 Page:946-956
ISSN:1755-098X
Container-title:Molecular Ecology Resources
language:en
Short-container-title:Mol Ecol Resour

Author:

Brown Shawn P.¹,Ferrer Astrid¹,Dalling James W.¹²,Heath Katy D.¹

Affiliation:

1. Department of Plant Biology University of Illinois at Urbana‐Champaign 505 S Goodwin Ave. Urbana IL 61801 USA

2. Smithsonian Tropical Research Institute Apartado 0843‐03092 Balboa Ancon Panama

Funder

National Science Foundation

Publisher

Wiley

Subject

Genetics,Ecology, Evolution, Behavior and Systematics,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/1755-0998.12507

Reference57 articles.

1. Quantifying microbial communities with 454 pyrosequencing: does read abundance count?

2. Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques

3. Comparison of ITS1 and ITS2 rDNA in 454 sequencing of hyperdiverse fungal communities

4. ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

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