‘Direct PCR’ optimization yields a rapid, cost-effective, nondestructive and efficient method for obtaining DNA barcodes without DNA extraction

Author:

Wong Wing Hing1,Tay Ywee Chieh1,Puniamoorthy Jayanthi1,Balke Michael23,Cranston Peter S.4,Meier Rudolf15

Affiliation:

1. Department of Biological Sciences; National University of Singapore; 14 Science Drive 4 Singapore 117543 Singapore

2. GeoBioCenter; Ludwig-Maximilians-University; Richard-Wagner-Str. 10 80333 Munich Germany

3. Zoological State Collection; Münchhausenstr 21 D-81247 Munich Germany

4. Evolution, Ecology and Genetics; Research School of Biology; The Australian National University; Canberra ACT 0200 Australia

5. University Scholars Programme; National University of Singapore; 14 Science Drive 4 Singapore 117543 Singapore

Funder

Public Utilities Board - Singapore

Publisher

Wiley

Subject

Genetics,Ecology, Evolution, Behavior and Systematics,Biotechnology

Reference73 articles.

1. Current state and perspectives of fungal DNA barcoding and rapid identification procedures;Begerow;Applied Microbiology and Biotechnology,2010

2. Occurrence and structural organization of the exocrine glands in the legs of ants;Billen;Arthropod Structure & Development,2009

3. Improving environmental and biodiversity monitoring in the Baltic Sea using DNA barcoding of Chironomidae (Diptera);Brodin;Molecular Ecology Resources,2013

4. The utility of DNA markers in classical taxonomy: using cytochrome oxidase 1 markers to differentiate Australian Cladopelma (Diptera: Chironomidae) midges;Carew;Annals of the Entomological Society of America,2005

5. DNA identification of urban Tanytarsini chironomids (Diptera: Chironomidae);Carew;Journal of the North America Benthological Society,2007

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