ITS all right mama: investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock communities of different complexities

Author:

Bjørnsgaard Aas Anders1,Davey Marie Louise1,Kauserud Håvard1

Affiliation:

1. Section for Genetics and Evolutionary Biology (Evogene); Department of Biosciences; University of Oslo; P.O. Box 1066 Blindern NO-0316 Oslo Norway

Funder

MLS-UiO

Publisher

Wiley

Subject

Genetics,Ecology, Evolution, Behavior and Systematics,Biotechnology

Reference56 articles.

1. The unite database for molecular identification of fungi-recent updates and future perspectives;Abarenkov;New Phytologist,2010

2. Effects of PCR cycle number and DNA polymerase type on the 16S rRNA gene pyrosequencing analysis of bacterial communities;Ahn;Journal of Microbiology,2012

3. At least 1 in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomalies;Ashelford;Applied and Environmental Microbiology,2005

4. ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases;Bellemain;BMC Microbiology,2010

5. Improved software detection and extraction of ITS1 and ITS2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data;Bengtsson-Palme;Methods in Ecology and Evolution,2013

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