Validation of dried blood spots for capturing hepatitis C virus diversity for genomic surveillance

Author:

Tully Damien C.12,Power Karen A.3,Sarette Jacklyn3,Stopka Thomas J.4,Friedmann Peter D.5,Korthuis P. Todd6,Cooper Hannah7,Young April M.8,Seal David W.9,Westergaard Ryan P.10,Allen Todd M.3

Affiliation:

1. Department of Infectious Disease Epidemiology London School of Hygiene and Tropical Medicine London UK

2. Center for Mathematical Modelling of Infectious Disease London School of Hygiene and Tropical Medicine London UK

3. Ragon Institute of MGH, MIT and Harvard Cambridge Massachusetts USA

4. Department of Public Health and Community Medicine Tufts University School of Medicine Boston Massachusetts USA

5. Office of Research, UMass Chan Medical School – Baystate Baystate Medical Center—University of Massachusetts Springfield Massachusetts USA

6. Oregon Health & Science University Portland Oregon USA

7. Rollins School of Public Health, Emory University Atlanta Georgia USA

8. University of Kentucky Lexington Kentucky USA

9. Tulane University, School of Public Health and Tropical Medicine New Orleans Louisiana USA

10. University of Wisconsin‐Madison Madison Wisconsin USA

Abstract

AbstractDried blood spots (DBS) have emerged as a promising alternative to traditional venous blood for hepatitis C virus (HCV) testing. However, their capacity to accurately reflect the genetic diversity of HCV remains poorly understood. We employed deep sequencing and advanced phylogenetic analyses on paired plasma and DBS samples from two common subtypes to evaluate the suitability of DBS for genomic surveillance. Results demonstrated that DBS captured equivalent viral diversity compared to plasma with no phylogenetic discordance observed. The ability of DBS to accurately reflect the profile of viral genetic diversity suggests it may be a promising avenue for future surveillance efforts to curb HCV outbreaks.

Funder

National Institute on Drug Abuse

Publisher

Wiley

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