Genome editing in rice using <scp>CRISPR</scp>/Cas12i3-Reference-Cited by-全球学者库

Genome editing in rice using CRISPR/Cas12i3

Published:2023-10-11 Issue:2 Volume:22 Page:379-385
ISSN:1467-7644
Container-title:Plant Biotechnology Journal
language:en
Short-container-title:Plant Biotechnology Journal

Author:

Lv Ping¹²,Su Fei¹²³,Chen Fangyuan¹²,Yan Chunxue¹²,Xia Dandan¹²,Sun Hui¹,Li Shanshan¹,Duan Zhiqiang¹,Ma Changle²^ORCID,Zhang Hui⁴^ORCID,Wang Mugui⁵,Niu Xiaomu¹,Zhu Jian‐Kang³⁶,Zhang Jinshan¹²^ORCID

Affiliation:

1. Bellagen Biotechnology Co. Ltd Ji'nan China

2. School of Life Sciences Shandong Normal University Ji'nan China

3. Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural Sciences Beijing China

4. College of Life Science Shanghai Normal University Shanghai China

5. National Nanfan Research Institute (Sanya) Chinese Academy of Agricultural Sciences Sanya China

6. Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and Technology Shenzhen China

Abstract

SummaryThe CRISPR/Cas type V‐I is a family of programmable nuclease systems that prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome‐editing application of Cas12i3, a type V‐I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3‐based Multiplex direct repeats (DR)‐spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide‐resistant rice plants using the base editors. These CRIPSR/Cas12i3‐based genome editing systems will facilitate precision molecular breeding in plants.

Funder

Key Technology Research and Development Program of Shandong Province

Publisher

Wiley

Subject

Plant Science,Agronomy and Crop Science,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.14192

Reference30 articles.

1. CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity

2. SnapShot: CRISPR-RNA-Guided Adaptive Immune Systems

3. Synergistic engineering of CRISPR‐Cas nucleases enables robust mammalian genome editing;Chen Y.;Innovation,2022

4. Multiplex Genome Engineering Using CRISPR/Cas Systems

5. Multigene Knockout Utilizing Off-Target Mutations of the CRISPR/Cas9 System in Rice

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