Affiliation:
1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection Academy of Agricultural Sciences Southwest University Chongqing China
2. Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education) Academy of Agricultural Sciences Southwest University Chongqing China
3. School of Biological and Environmental Sciences Liverpool John Moores University Liverpool UK
Abstract
AbstractInsecticide resistance in Panonychus citri is a major obstacle to mite control in citrus orchards. Pyrethroid insecticides are continually used to control mites in China, although resistance to pyrethroids has evolved in some populations. Here, the resistance to the pyrethroid fenpropathrin was investigated and 7 out of 8 field‐collected populations of P. citri exhibited a high level of resistance, ranging from 171‐fold to 15 391‐fold higher than the susceptible (SS) comparison strain. Three voltage‐gated sodium channel (VGSC) mutations were identified in the tested populations: L1031V, F1747L, and F1751I. Amplicon sequencing was used to evaluate the frequency of these mutations in the 19 field populations. L1031V and F1751I were present in all populations at frequencies of 11.6%–82.1% and 0.5%–31.8%, respectively, whereas the F1747L mutation was only present in 12 populations from Chongqing, Sichuan, Guangxi, and Yunnan provinces. Introduction of these mutations singly or in combination into transgenic flies significantly increased their resistance to fenpropathrin and these flies also exhibited reduced mortality after exposure to the pyrethroids permethrin and β‐cypermethrin. Panonychus citri VGSC homology modeling and ligand docking indicate that F1747 and F1751 form direct binding contacts with pyrethroids, which are lost with mutation, whereas L1031 mutation may diminish pyrethroid effects through an allosteric mechanism. Overall, the results provide molecular markers for monitoring pest resistance to pyrethroids and offer new insights into the basis of pyrethroid actions on sodium channels.
Funder
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities
Subject
Insect Science,General Biochemistry, Genetics and Molecular Biology,Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献