Diagnostic characterization of respiratory allergies by means of a multiplex immunoassay

Author:

Millen J L M12,Willems I12,Slingers G134,Raes M34,Koppen G1,Langie S A S125ORCID

Affiliation:

1. VITO – Health, Mol, Belgium

2. Centre for Environmental Sciences, Hasselt University, Diepenbeek, Belgium

3. Faculty of Medicine and Life Sciences, UHasselt, LCRC, Diepenbeek, Belgium

4. Paediatrics, Jessa Hospital, Hasselt, Belgium

5. Department of Pharmacology and Toxicology, School for Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, Maastricht, the Netherlands

Abstract

Summary Allergic sensitization is commonly assessed in patients by performing the skin prick test (SPT) or determining specific immunoglobulin (IgE) levels in blood samples with the ImmunoCAP™ assay, which measures each allergen and sample separately. This paper explores the possibility to investigate respiratory allergies with a high throughput method, the Meso Scale Discovery (MSD) multiplex immunoassay, measuring IgE levels in low volumes of blood. The MSD multiplex immunoassay, developed and optimized with standards and allergens from Radim Diagnostics, was validated against the SPT and the ImmunoCAP assay. For 18 adults (15 respiratory allergy patients and three controls), blood collection and the SPT were performed within the same hour. Pearson correlations and Bland–Altman analysis showed high comparability of the MSD multiplex immunoassay with the SPT and the ImmunoCAP assay, except for house dust mite. The sensitivity of the MSD multiplexed assay was ≥78% for most allergens compared to the SPT and ImmunoCAP assay. Additionally, the specificity of the MSD multiplex immunoassay was ≥ 87% – the majority showing 100% specificity. Only the rye allergen had a low specificity when compared to the SPT, probably due to cross-reactivity. The reproducibility of the MSD multiplex immunoassay, assessed as intra- and interassay reproducibility and biological variability between different sampling moments, showed significantly high correlations (r = 0·943–1) for all tested subjects (apart from subject 13; r = 0·65–0·99). The MSD multiplex immunoassay is a reliable method to detect specific IgE levels against respiratory allergens in a multiplexed and high-throughput manner, using blood samples as small as from a finger prick.

Funder

Fonds Wetenschappelijk Onderzoek

Publisher

Oxford University Press (OUP)

Subject

Immunology,Immunology and Allergy

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Optical Glass Microsphere Enabled Rapid Single‐Molecular Fluoroimmunoassay;Advanced Optical Materials;2023-04-24

2. EAACIMolecular Allergology User's Guide 2.0;Pediatric Allergy and Immunology;2023-03

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