Affiliation:
1. Department of Plant Pathology, Entomology, and Microbiology Iowa State University Ames IA 50011 USA
2. Department of Biology Duke University Durham NC 27708 USA
3. Howard Hughes Medical Institute Duke University Durham NC 27708 USA
Abstract
Summary
Single‐cell proteomics (SCP) is an emerging approach to resolve cellular heterogeneity within complex tissues of multi‐cellular organisms.
Here, we demonstrate the feasibility of SCP on plant samples using the model plant Arabidopsis thaliana. Specifically, we focused on examining isolated single cells from the cortex and endodermis, which are two adjacent root cell types derived from a common stem cell lineage.
From 756 root cells, we identified 3763 proteins and 1118 proteins/cell. Ultimately, we focus on 3217 proteins quantified following stringent filtering. Of these, we identified 596 proteins whose expression is enriched in either the cortex or endodermis and are able to differentiate these closely related plant cell types.
Collectivity, this study demonstrates that SCP can resolve neighboring cell types with distinct functions, thereby facilitating the identification of biomarkers and candidate proteins to enable functional genomics.
Funder
Plant Sciences Institute, Iowa State University
Howard Hughes Medical Institute
Division of Integrative Organismal Systems
National Institute of Food and Agriculture
Cited by
2 articles.
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