Bacillus aryabhattai enhanced proline content, stabilized membrane and improved growth of cowpea under NaCl-induced salinity stress

Abstract

Abstract Aims Salinity stress affects the growth of cowpea particularly at the stages of seed germination and early vegetative growth. This study examined the potential of particular stress-tolerant rhizospheric bacteria to improve the growth of cowpea under conditions of salinity stress. Methods and Results Two rhizobacillus genotypes, Bacillus filamentosus-C8 and Bacillus aryabhattai-C29 were evaluated for their potentials to protect cowpea under NaCl-induced salinity stress. At 200 mM of NaCl concentration, control (non-inoculated) cowpea was affected, C8 was not able to significantly (p ≤ 0.05) alleviate the effects of salinity stress on cowpea growth while C29 significantly (p ≤ 0.05) reduced leaf wilting, increased chlorophyll content and improved the growth of cowpea plant under stressed condition. Interestingly, C29 significantly (p ≤ 0.05) induced high proline content and stabilized membrane by loss of electrolytes. Conclusion Our results indicate that stabilized membrane and enhanced proline content by Bacillus aryabhattai-C29 supported the growth of cowpea under salinity stress condition. Significance and Impact of the Study This study revealed that rhizospheric bacteria screened for salinity stress tolerant have potential to be used as an effective bioprotectant for sustainable growth of cowpea under salinity stress condition.