Affiliation:
1. MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology, Huazhong Agricultural University Wuhan Hubei Province China
2. College of Agriculture Jinhua Polytechnic Jinhua Zhejiang Province China
3. Xiangyang Academy of Agricultural Sciences Xiangyang Hubei China
4. Tai'an Academy of Agricultural Sciences Tai'an Shandong China
Abstract
AbstractShading significantly affects rapeseed yield, while reasonable nitrogen (N) application has efficiency gains. However, the functions and mechanisms of N are not fully established for shaded rapeseed plants. Therefore, we conducted a 2‐year field experiment to study the effect of N on pod wall morphology and carbon metabolism of shaded rapeseed. Two varieties, three N rates (120 [N1], 240 [N2], and 360 [N3] kg hm−2) and two light intensities (100 and 70% light transmission) from 10 to 35 days after the end of flowering were set as experimental parameters. Shading decreased the pod wall chlorophyll content, ribulose 1,5‐bisphosphate carboxylase (Rubisco) activity and glucose content at 25 and 35 days after flowering (DAF). Decreased sucrose synthase (SuSy) and sucrose phosphate synthase activity caused by shading reduced sucrose and fructose content. They are responsible for the decline in the 1000‐seed weight and a 22.1–37.6% decline in seed yield. More N under shading promoted pod elongation and pigment content, improved chloroplast ultrastructure, increased Rubisco and SuSy activity at 35 DAF, thus contributing to pod wall photosynthesis and fructose and glucose levels in shaded rapeseed plants. Similar trends were observed in pod number, pod weight, and seed weight, while the greatest increase in seed/wall ratio was observed under N2 for shaded rapeseed plants. The results indicated that N can reduce the yield difference between different light conditions and balance partitioning and conversion of photoassimilates in pod wall, but avoid applying an excessive amount of nitrogen.
Funder
Earmarked Fund for China Agriculture Research System
National Natural Science Foundation of China
Subject
Cell Biology,Plant Science,Genetics,General Medicine,Physiology
Cited by
2 articles.
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