Increased Sensitivity of Cholera Toxin Detection by Enzyme-Linked Immunosorbent Assay with Chemical Immobilization of Antibody onto Nylon Surface Providing Hydrophobicity
Author:
Affiliation:
1. Medical Products Division; Unitika Ltd.; Okazaki Aichi 444-8511 Japan
2. Department of Bacterial Infections, Research Institute for Microbial Diseases; Osaka University; Suita Osaka 565-0871 Japan
Publisher
Wiley
Subject
Virology,Immunology,Microbiology
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1348-0421.1999.tb01235.x/fullpdf
Reference14 articles.
1. ELISA solid phase: partial denaturation of coating antibody yields a more efficient solid phase;Conradie;J. Immunol. Methods,1983
2. Immobilization of antibodies on nylon for use in enzyme-linked immunoassay;Hendry;J. Immunol. Methods,1980
3. Control of immunoglobulin G adsorbed by designing the characters of polymer surface;Hiraoka;Kobunshi Ron-bunshu,1985
4. A novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two Vibrio parahaemolyticus toxins in a modified enzyme-linked immunosorbent assay;Honda;Clin. Diagn. Lab. Immunol.,1995
5. Production of monoclonal antibodies against thermostable direct hemolysin of Vibrio parahaemolyticus and application of the antibodies for enzyme-linked immunosorbent assay;Honda;Med. Microbiol. Immunol.,1989
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