Two‐dimensional Polyacrylamide Gel Electrophoresis of Human Hair Matrix Proteins Using Isoelectric Focusing Electrophoresis as the First Dimension

Abstract

AbstractS‐carboxymethylated (SCM) matrix proteins from fifteen normal human scalp hair specimens were analyzed using two‐dimensional polyacrylamide gel electrophoresis (PAGE) with usual isoelectric focusing electrophoresis as the first dimension and then with usual sodium dodecyl sulfate‐PAGE as the second dimension. On the electrophoregrams obtained, one low‐mobility smear of SCM proteins embedding one SCM protein band was always seen. Many higher‐mobility SCM protein spots were also always seen. However, some variations were seen in these SCM protein compositions; in type I, there were eighteen SCM protein components and, in type II, seventeen components.