The Assay of Enzyme Activity by the Plate-Diffusion Technique

Abstract

Abstract The technique described provides a convenient means for estimating various enzymes quantitatively. Only a few examples are quoted, but provided suitable indicator substrates can be found the range can be extended. Having established the procedure for one enzyme, it is applicable to others, subject only to the variation in substrate required and the different dilutions of the enzyme preparation necessary to obtain satisfactory responses. Thus, the method is more convenient and easier to manipulate than are the existing methods, including those official in the B.P. and U.S.P.; moreover, as already stated, the assay is quantitative, the limits of error being only about 8 per cent (P = 0.95).