Affiliation:
1. Department of Pharmacology, University of California, San Francisco Medical Center, San Francisco, California
Abstract
Abstract
The sensitive and specific fluorimetric methods introduced by Jensen (1952; 1953) for digitalis estimation were re-evaluated for the assay of cardiotonic steroids and other steroids of biological interest. Characteristics of the excitation and fluorescence spectra were determined. Using a modification of Jensen's original digitoxin method and a spectrofluorometer, a linear relation was demonstrated between fluorescence intensity and concentrations from 0.01 to 6 μg./ml. for both digitoxin and digoxin. Application of these methods to the assay of gitoxin and cholesterol would seem to be equally practicable. In addition, scillaren A and B and strophanthin K can be made to fluoresce weakly. On the other hand, ouabain, cholestanol, testosterone, and hydrocortisone showed no fluorescence. Possible mechanisms of the reactions are discussed
Publisher
Oxford University Press (OUP)
Subject
Pharmaceutical Science,Pharmacology
Cited by
55 articles.
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