In-vitro Evaluation of 5-Lipoxygenase and Cyclo-oxygenase Inhibitors using Bovine Neutrophils and Platelets and HPLC-Reference-Cited by-同舟云学术

In-vitro Evaluation of 5-Lipoxygenase and Cyclo-oxygenase Inhibitors using Bovine Neutrophils and Platelets and HPLC

Published:1992-05 Issue:5 Volume:44 Page:419-424
ISSN:2042-7158
Container-title:Journal of Pharmacy and Pharmacology
language:en
Short-container-title:

Author:

Dannhardt Gerd¹,Lehr Matthias¹

Affiliation:

1. Institut für Pharmazeutische Chemie der Johann Wolfgang Goethe-Universitäl, Georg-Voigt-Str.14, 6000 Frankfurt, Germany

Abstract

Abstract Inhibition of 5-lipoxygenase has been determined by monitoring the formation of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in bovine polymorphonuclear leucocytes. For evaluating the inhibition of cyclo-oxygenase two different test systems are presented: the first uses 12-hydroxyheptadecatrienoic acid produced by bovine platelets as an indicator of the cyclo-oxygenase activity; the second test system monitors the prostaglandin E2 formation by bovine platelets. All arachidonic acid metabolites are quantified by reverse-phase HPLC with UV-detection.

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

Link

http://academic.oup.com/jpp/article-pdf/44/5/419/37244024/j.2042-7158.1992.tb03636.x.pdf

Reference38 articles.

1. Prostaglandins, Leukotrienes, and Lipoxins

2. Inhibition of prostaglandin production in cultured makrophages by anti-inflammatory drugs;Brune;Naunyn Schmiedebergs Arch. Pharmacol.,1981

3. Novel 1H-benz-imidazol-4-ols with potent 5-lipoxygenase inhibitory activity;Buckle;J. Med. Chem.,1987

4. Arylmethyl Phenyl Ethers

5. Untersuchungen zur Reaktion isomerer Diaryldihydro-pyrrolizine mit 2,2-Dimethyl-1,3-dioxan-4,6-dion (meldrums' acid);Dannhardt;Chemiker-Zeitung,1990

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