Covalent binding of antibodies to liposomes using a novel lipid derivative-Reference-Cited by-同舟云学术

Covalent binding of antibodies to liposomes using a novel lipid derivative

Published:1984-07 Issue:7 Volume:36 Page:465-466
ISSN:2042-7158
Container-title:Journal of Pharmacy and Pharmacology
language:en
Short-container-title:

Author:

Goundalkar Ashok¹,Ghose Tarun²,Mezei Michael¹

Affiliation:

1. College of Pharmacy, Faculty of Health Professions

2. Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax, N.S. Canada B3H 3J5

Abstract

Abstract N-[3−(2−Pyridyldithio) propionyl] stearylamine (PDP-SA) was synthesized from a reaction between stearylamine and the heterobifunctional reagent N-succinimidyl−3−(2−pyridyldithio) propionate. Use of this PDP-SA to covalently couple antibodies to liposomes was investigated. The binding efficiency was found to be 24–32%. The antibodies bound to liposomes were shown to retain the specific antibody activity. This new procedure of coupling antibodies to lipsosomes could be an efficient means to deliver drugs to selected target organs, especially in cancer chemotherapy.

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

Link

http://academic.oup.com/jpp/article-pdf/36/7/465/37468511/j.2042-7158.1984.tb04427.x.pdf

Reference11 articles.

1. Monoclonal antibody covalently coupled to liposomes: Specific targeting to cells

2. Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent

3. Antibody coating of liposomes with 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimide and the effect on target specificity

4. Immobilization of protein molecules on liposomes

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